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rotem ® delta rotational thromboelastometric system  (TEM International)

 
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    TEM International rotem ® delta rotational thromboelastometric system
    Effect of adrenaline on the kinetics of platelet-fibrin clot formation. Whole blood samples were incubated with or without rauwolscine (10 μM, for 10 min) followed by the addition of adrenaline (for 2 min) and collagen (150 ng/ml) with initial stirring (30 s). After 10 min of incubation, at room temperature, samples were analyzed toward kinetics of clot formation using <t>rotational</t> <t>thromboelastometry.</t> Clotting was initiated by recalcification (12 mM CaCl 2 final conc.). Control samples were without any addition. Representative coagulation profiles and records of clot formation rate (CFR) for 6 experiments are presented in (A) . (B–D) Parameters associated with clotting initiation (CT, clotting time; CFT, clot formation time), propagation (alpha angle; MaxV, maximal velocity of clot formation; MaxV-t, time to reach maximal velocity of clotting), and stabilization (MCF, maximum clot firmness; G, shear modulus strength) were measured. Data are means ± S.D. from 6 experiments. Parameters value range in control was: CT: 215–667 s; CFT: 79–176 s; Alpha: 64–74°; MaxV: 12–18 mm*100/s; MaxV-t: 351–855 s; MCF: 65–75 mm; G: 936–1,492. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. adrenaline 10 nM + coll. (E) Whole blood samples (supplemented with AF488-fibrinogen to visualize fibrin formation) were incubated without any addition (control) or with adrenaline (for 2 min) or rauwolscine (10 μM, for 10 min) + adrenaline (for 2 min). Kinetics of fibrin formation within thrombi, formed under flow (1,000 s – 1 ) on collagen-coated surfaces, was recorded in confocal microscope. The fibrin(ogen)-associated fluorescence was recorded and expressed as% of maximal fluorescence obtained (considered as 100%) vs. time. Data are means ± S.D. from 4 experiments. * p < 0.05 vs. control; # p < 0.05 vs. adrenaline 1 nM.
    Rotem ® Delta Rotational Thromboelastometric System, supplied by TEM International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rotem+%C2%AE+delta+rotational+thromboelastometric+system/rotem1/pmc08134743-85-5-11
    Average 90 stars, based on 1 article reviews
    rotem ® delta rotational thromboelastometric system - by Bioz Stars, 2026-10
    90/100 stars

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    1) Product Images from "Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis"

    Article Title: Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis

    Journal: Frontiers in Physiology

    doi: 10.3389/fphys.2021.657881

    Effect of adrenaline on the kinetics of platelet-fibrin clot formation. Whole blood samples were incubated with or without rauwolscine (10 μM, for 10 min) followed by the addition of adrenaline (for 2 min) and collagen (150 ng/ml) with initial stirring (30 s). After 10 min of incubation, at room temperature, samples were analyzed toward kinetics of clot formation using rotational thromboelastometry. Clotting was initiated by recalcification (12 mM CaCl 2 final conc.). Control samples were without any addition. Representative coagulation profiles and records of clot formation rate (CFR) for 6 experiments are presented in (A) . (B–D) Parameters associated with clotting initiation (CT, clotting time; CFT, clot formation time), propagation (alpha angle; MaxV, maximal velocity of clot formation; MaxV-t, time to reach maximal velocity of clotting), and stabilization (MCF, maximum clot firmness; G, shear modulus strength) were measured. Data are means ± S.D. from 6 experiments. Parameters value range in control was: CT: 215–667 s; CFT: 79–176 s; Alpha: 64–74°; MaxV: 12–18 mm*100/s; MaxV-t: 351–855 s; MCF: 65–75 mm; G: 936–1,492. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. adrenaline 10 nM + coll. (E) Whole blood samples (supplemented with AF488-fibrinogen to visualize fibrin formation) were incubated without any addition (control) or with adrenaline (for 2 min) or rauwolscine (10 μM, for 10 min) + adrenaline (for 2 min). Kinetics of fibrin formation within thrombi, formed under flow (1,000 s – 1 ) on collagen-coated surfaces, was recorded in confocal microscope. The fibrin(ogen)-associated fluorescence was recorded and expressed as% of maximal fluorescence obtained (considered as 100%) vs. time. Data are means ± S.D. from 4 experiments. * p < 0.05 vs. control; # p < 0.05 vs. adrenaline 1 nM.
    Figure Legend Snippet: Effect of adrenaline on the kinetics of platelet-fibrin clot formation. Whole blood samples were incubated with or without rauwolscine (10 μM, for 10 min) followed by the addition of adrenaline (for 2 min) and collagen (150 ng/ml) with initial stirring (30 s). After 10 min of incubation, at room temperature, samples were analyzed toward kinetics of clot formation using rotational thromboelastometry. Clotting was initiated by recalcification (12 mM CaCl 2 final conc.). Control samples were without any addition. Representative coagulation profiles and records of clot formation rate (CFR) for 6 experiments are presented in (A) . (B–D) Parameters associated with clotting initiation (CT, clotting time; CFT, clot formation time), propagation (alpha angle; MaxV, maximal velocity of clot formation; MaxV-t, time to reach maximal velocity of clotting), and stabilization (MCF, maximum clot firmness; G, shear modulus strength) were measured. Data are means ± S.D. from 6 experiments. Parameters value range in control was: CT: 215–667 s; CFT: 79–176 s; Alpha: 64–74°; MaxV: 12–18 mm*100/s; MaxV-t: 351–855 s; MCF: 65–75 mm; G: 936–1,492. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. adrenaline 10 nM + coll. (E) Whole blood samples (supplemented with AF488-fibrinogen to visualize fibrin formation) were incubated without any addition (control) or with adrenaline (for 2 min) or rauwolscine (10 μM, for 10 min) + adrenaline (for 2 min). Kinetics of fibrin formation within thrombi, formed under flow (1,000 s – 1 ) on collagen-coated surfaces, was recorded in confocal microscope. The fibrin(ogen)-associated fluorescence was recorded and expressed as% of maximal fluorescence obtained (considered as 100%) vs. time. Data are means ± S.D. from 4 experiments. * p < 0.05 vs. control; # p < 0.05 vs. adrenaline 1 nM.

    Techniques Used: Incubation, Coagulation, Microscopy, Fluorescence

    Related Articles

    Incubation:

    Article Title: Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis
    Article Snippet: Thromboelastometric measurements were performed using ROTEM ® Delta rotational thromboelastometric system (Tem International GmbH, Manheim, Germany) ( ). .. W

    Coagulation:

    Article Title: Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis
    Article Snippet: Thromboelastometric measurements were performed using ROTEM ® Delta rotational thromboelastometric system (Tem International GmbH, Manheim, Germany) ( ). .. W

    Microscopy:

    Article Title: Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis
    Article Snippet: Thromboelastometric measurements were performed using ROTEM ® Delta rotational thromboelastometric system (Tem International GmbH, Manheim, Germany) ( ). .. W

    Fluorescence:

    Article Title: Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis
    Article Snippet: Thromboelastometric measurements were performed using ROTEM ® Delta rotational thromboelastometric system (Tem International GmbH, Manheim, Germany) ( ). .. W



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    rotem ® delta rotational thromboelastometric system  (TEM International)
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    TEM International rotem ® delta rotational thromboelastometric system
    Effect of adrenaline on the kinetics of platelet-fibrin clot formation. Whole blood samples were incubated with or without rauwolscine (10 μM, for 10 min) followed by the addition of adrenaline (for 2 min) and collagen (150 ng/ml) with initial stirring (30 s). After 10 min of incubation, at room temperature, samples were analyzed toward kinetics of clot formation using <t>rotational</t> <t>thromboelastometry.</t> Clotting was initiated by recalcification (12 mM CaCl 2 final conc.). Control samples were without any addition. Representative coagulation profiles and records of clot formation rate (CFR) for 6 experiments are presented in (A) . (B–D) Parameters associated with clotting initiation (CT, clotting time; CFT, clot formation time), propagation (alpha angle; MaxV, maximal velocity of clot formation; MaxV-t, time to reach maximal velocity of clotting), and stabilization (MCF, maximum clot firmness; G, shear modulus strength) were measured. Data are means ± S.D. from 6 experiments. Parameters value range in control was: CT: 215–667 s; CFT: 79–176 s; Alpha: 64–74°; MaxV: 12–18 mm*100/s; MaxV-t: 351–855 s; MCF: 65–75 mm; G: 936–1,492. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. adrenaline 10 nM + coll. (E) Whole blood samples (supplemented with AF488-fibrinogen to visualize fibrin formation) were incubated without any addition (control) or with adrenaline (for 2 min) or rauwolscine (10 μM, for 10 min) + adrenaline (for 2 min). Kinetics of fibrin formation within thrombi, formed under flow (1,000 s – 1 ) on collagen-coated surfaces, was recorded in confocal microscope. The fibrin(ogen)-associated fluorescence was recorded and expressed as% of maximal fluorescence obtained (considered as 100%) vs. time. Data are means ± S.D. from 4 experiments. * p < 0.05 vs. control; # p < 0.05 vs. adrenaline 1 nM.
    Rotem ® Delta Rotational Thromboelastometric System, supplied by TEM International, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rotem+%C2%AE+delta+rotational+thromboelastometric+system/rotem1/pmc08134743-85-5-11
    Average 90 stars, based on 1 article reviews
    rotem ® delta rotational thromboelastometric system - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Effect of adrenaline on the kinetics of platelet-fibrin clot formation. Whole blood samples were incubated with or without rauwolscine (10 μM, for 10 min) followed by the addition of adrenaline (for 2 min) and collagen (150 ng/ml) with initial stirring (30 s). After 10 min of incubation, at room temperature, samples were analyzed toward kinetics of clot formation using rotational thromboelastometry. Clotting was initiated by recalcification (12 mM CaCl 2 final conc.). Control samples were without any addition. Representative coagulation profiles and records of clot formation rate (CFR) for 6 experiments are presented in (A) . (B–D) Parameters associated with clotting initiation (CT, clotting time; CFT, clot formation time), propagation (alpha angle; MaxV, maximal velocity of clot formation; MaxV-t, time to reach maximal velocity of clotting), and stabilization (MCF, maximum clot firmness; G, shear modulus strength) were measured. Data are means ± S.D. from 6 experiments. Parameters value range in control was: CT: 215–667 s; CFT: 79–176 s; Alpha: 64–74°; MaxV: 12–18 mm*100/s; MaxV-t: 351–855 s; MCF: 65–75 mm; G: 936–1,492. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. adrenaline 10 nM + coll. (E) Whole blood samples (supplemented with AF488-fibrinogen to visualize fibrin formation) were incubated without any addition (control) or with adrenaline (for 2 min) or rauwolscine (10 μM, for 10 min) + adrenaline (for 2 min). Kinetics of fibrin formation within thrombi, formed under flow (1,000 s – 1 ) on collagen-coated surfaces, was recorded in confocal microscope. The fibrin(ogen)-associated fluorescence was recorded and expressed as% of maximal fluorescence obtained (considered as 100%) vs. time. Data are means ± S.D. from 4 experiments. * p < 0.05 vs. control; # p < 0.05 vs. adrenaline 1 nM.

    Journal: Frontiers in Physiology

    Article Title: Adrenaline May Contribute to Prothrombotic Condition via Augmentation of Platelet Procoagulant Response, Enhancement of Fibrin Formation, and Attenuation of Fibrinolysis

    doi: 10.3389/fphys.2021.657881

    Figure Lengend Snippet: Effect of adrenaline on the kinetics of platelet-fibrin clot formation. Whole blood samples were incubated with or without rauwolscine (10 μM, for 10 min) followed by the addition of adrenaline (for 2 min) and collagen (150 ng/ml) with initial stirring (30 s). After 10 min of incubation, at room temperature, samples were analyzed toward kinetics of clot formation using rotational thromboelastometry. Clotting was initiated by recalcification (12 mM CaCl 2 final conc.). Control samples were without any addition. Representative coagulation profiles and records of clot formation rate (CFR) for 6 experiments are presented in (A) . (B–D) Parameters associated with clotting initiation (CT, clotting time; CFT, clot formation time), propagation (alpha angle; MaxV, maximal velocity of clot formation; MaxV-t, time to reach maximal velocity of clotting), and stabilization (MCF, maximum clot firmness; G, shear modulus strength) were measured. Data are means ± S.D. from 6 experiments. Parameters value range in control was: CT: 215–667 s; CFT: 79–176 s; Alpha: 64–74°; MaxV: 12–18 mm*100/s; MaxV-t: 351–855 s; MCF: 65–75 mm; G: 936–1,492. * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. control; # p < 0.05, ## p < 0.01, and ### p < 0.001 vs. adrenaline 10 nM + coll. (E) Whole blood samples (supplemented with AF488-fibrinogen to visualize fibrin formation) were incubated without any addition (control) or with adrenaline (for 2 min) or rauwolscine (10 μM, for 10 min) + adrenaline (for 2 min). Kinetics of fibrin formation within thrombi, formed under flow (1,000 s – 1 ) on collagen-coated surfaces, was recorded in confocal microscope. The fibrin(ogen)-associated fluorescence was recorded and expressed as% of maximal fluorescence obtained (considered as 100%) vs. time. Data are means ± S.D. from 4 experiments. * p < 0.05 vs. control; # p < 0.05 vs. adrenaline 1 nM.

    Article Snippet: Thromboelastometric measurements were performed using ROTEM ® Delta rotational thromboelastometric system (Tem International GmbH, Manheim, Germany) ( ).

    Techniques: Incubation, Coagulation, Microscopy, Fluorescence